51社区黑料

Synopsis

The gold standard in chemical synthesis of nucleic acids鈥擠NA and RNA鈥攖ypically yields only one oligonucleotide sequence per run, followed by labor-intensive downstream analysis. This double bottleneck is partially addressed by microarray technology, which enables rapid, discrete analysis of thousands of pre-synthesized oligonucleotides. However, these oligonucleotides are still produced in a low-throughput manner and then immobilized on individual surface-bound features, forming structured libraries, or microarrays, that can be analyzed simultaneously. 鹿^, 虏

To overcome the bottleneck of synthesis itself, spatially controlled in situ synthesis has been developed. Here, photolithographic synthesis stands out as the most robust鈥攁nd currently the only high-throughput鈥攁pproach for fabricating both DNA and RNA oligonucleotides. 虏^, 鲁

This talk will showcase how we advanced the photolithographic synthesis of RNA and how it can be harnessed to generate highly reproducible, high-density oligonucleotide microarrays. It will cover applications in systematic RNA aptamer screening 虏, in probing sequence-dependence of fluorescence dyes 鈦�^, 鈦�, and in storage of digital data through complementary hybridization. 鲁^, 鈦�

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